• Cytochrome P450 enzyme regulation
• Drug metabolism
• Environmental toxicology
• Chemical and hormonal carcinogenesis
• Bile acid metabolism

Title: Professor

Email: bandiera@interchange.ubc.ca

Telephone: 604-822-3815

Fax: 604-822-3035

Degrees:

 

Research Focus:

The cytochrome P450 (CYP) enzymes are a major focus of research in my laboratory. Projects include studies of (a) the mechanisms by which hormones regulate expression of CYP enzymes, (b) the effects of exposure to xenobiotics on CYP-mediated activities and protein levels, and (c) the role of individual enzymes in the metabolism of drugs, physiological compounds, and environmental chemicals. A second focus of research is environmental toxicology. Research in this area includes investigations of the toxicological and biochemical effects of halogenated compounds, including polychlorinated biphenyls and pesticides, on humans and wildlife.

Current Projects:

Hormonal regulation of CYP expression We are examining the role of pituitary and gonadal hormones in regulating CYP enzyme expression in liver and extrahepatic tissues. Hepatic bile acid biotransformation Bile acids have important functions in the body. We are interested in identifying the pathways by which bile acids are biotransformed in liver and to determine the enzymes involved in metabolite formation. Studies involve detection and quantification of hepatic bile acid metabolites by liquid chromatography-mass spectrometry. Hepatic metabolism of environmental contaminants Environmental contaminant composition differs among ecosystems and contaminants affect species inhabiting these ecosystems differently. To determine why animal species differ with respect to the type and amount of contaminants found in their tissues and in their susceptibility to adverse health effects, we are studying the metabolism of contaminants such as PCBs and PBDEs using hepatic microsomal biotransformation assays. Structure-activity analysis of toxic and biochemical effects of environmental contaminants We are examining the influence of halogen number and position, in compounds such as PCBs and PBDEs, as determinants of their interaction with CYP enzymes and their toxicity.

Selected Publications:

Deo, A.K. and Bandiera, S.M. 3-Ketocholanoic acid is the major in vitro human hepatic microsomal metabolite of lithocholic acid. Drug Metabolism and Disposition 37: in press (2009). Deb, S. and Bandiera, S.M. Characterization and expression of extrahepatic CYP2S1. Expert Opinion on Drug Metabolism and Toxicology 5(4):367-380 (2009). Leung, G.S., Kawai, M., Tai, J.K., Chen, J., Bandiera, S.M. and Chang, T.K.H., Developmental expression and endocrine regulation of CYP1B1 in rat testis. Drug Metabolism and Disposition 37:1-6 (2009). Deo, A.K. and Bandiera, S.M. Identification of human hepatic cytochrome P450 enzymes involved in the biotransformation of cholic and chenodeoxycholic acids. Drug Metabolism and Disposition 36:1983-1991 (2008). Deo, A.K. and Bandiera, S.M. Involvement of multiple cytochrome P450 enzymes in the biotransformation of lithocholic acid by rat hepatic microsomes. Drug Metabolism and Disposition 36:442-451 (2008). Kania-Korwel, I., Hrycay, E.G., Bandiera, S.M. and Lehmler, H-J. 2,2',3,3',6,6' -Hexachlorobiphenyl (PCB 136) atropisomers interact enantioselectively with hepatic microsomal cytochrome P450 enzymes. Chemical Research in Toxicology 21:1295-1303 (2008). Hrycay, E.G. and Bandiera, S.M. Cytochrome P450 Enzymes. In: Preclinical Development Handbook: ADME and Biopharmaceutical Properties (S.C. Gad, ed.), John Wiley and Sons, Inc., Hoboken, NJ, pp. 627-696 (2007). Edwards, P.R., Hrycay, E.G. and Bandiera, S.M. Differential inhibition of hepatic microsomal alkoxyresorufin O-dealkylation activities by tetrachlorobiphenyls. Chemico-Biological Interactions 169:42-52 (2007). Kelly, B.C., Gray, S.L., Ikonomou, M.G., Macdonald, J.S., Bandiera, S.M. and Hrycay, E.G. Lipid reserve dynamics and magnification of persistent organic pollutants in spawning sockeye salmon (Oncorhynchus nerka) from the Fraser River, British Columbia. Environmental Science and Technology 41:3083-3089 (2007). Assuncao, M.G.L., Miller, K.A., Dangerfield, N.J., Bandiera, S.M. and Ross, P.S. Cytochrome P450 1A expression and organochlorine contaminants in harbour seals (Phoca vitulina): Evaluating a biopsy approach. Comparative Biochemistry and Physiology - Part C, Toxicology and Pharmacology 145:256-264 (2007). Bandiera, S.M. Reproductive and endocrine effects of p-nonylphenol and methoxychlor: A review. Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry 6(1):15-26 (2006).